Laboratory Techniques

Page content: Department/Institution Offering Unit | Unit Points | Contacts | Overall Objectives | Dates and Contact Hours | Content | Assessment Breakdown

Department/Institution Offering Unit

Clinical Neurosciences Laboratories
St. Vincent's Hospital
Level 5, 41 Victoria Parade
Fitzroy
Victoria 3065 Australia

Unit Points

Research Points: N/A

Coursework Points: N/A, but successful completion of the course is a hurdle requirement.

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Contacts

Unit Coordinator/s:

Dr Robert Kapsa
Clinical Neurosciences Laboratories
St. Vincent's Hospital
Level 5, 41 Victoria Parade
Fitzroy Vic 3065

tel: +61 3 9288 3344
email: RKAPSA@bionicear.org

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Dates and Contact Hours

4 Day Workshop: Tues 14th - Fri 17th July 2009

10.00am - 4.30pm every day

Please report to

5th Floor, Daly Wing
35 Victoria Pde
Fitzroy

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Overall Objectives

To provide a general introduction to the main techniques used in a molecular laboratory.

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Content

1. Use of Micropipettes
   • Accuracy and Reproducibility Testing
2. Liquid Sample Manipulation:
   • Dilutions: Standard and Serial
   • Protein Content Assay by Spectrophotometry
   • Preparation of Solutions: Introduction to Concentration Calculations, Stock and Working Solutions
3. DNA (plasmid) Extraction
4. Agarose Gel Electrophoresis
5. Restriction Enzyme Digestion of DNA
6. Polymerase Chain Reaction (PCR)

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Assessment Breakdown

Research Component: N/A

Coursework Component: Hurdle requirement; to be announced.

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